Gain the confidence to move forward with informative controlsWhile lentiviral transduction can be a powerful and effective method for delivering expression cassettes to target cells, enabling stable expression of genes, miRNAs, shRNAs, etc., some cell lines are transduced less efficiently than others. To help with lentiviral transduction optimization and troubleshooting, SBI offers a series of positive transduction controls that come as pre-packaged, ready-to-transduce lentiviral particles as well as ready-to-transfect and package lentivector plasmids. The pSIH1-H1-siLuc-copGFP Positive Transduction Control enables quantitation of HIV-based lentiviral transduction efficiency using the bright copGFP protein.

• Easily visualize and quantify transduction efficiency with copGFP
• Optimize transduction conditions for your cell line
• Test the sensitivity of target cells to HIV-based lentiviral transduction

pSIH1-H1-siLuc-copGFP expresses the full-length copGFP gene with human-optimized codons from the CMV promoter for high levels of expression in mammalian cells. The copGFP marker is a novel natural green monomeric GFP-like protein from copepods (Pontellina sp.). The copGFP protein is a non-toxic, non-aggregating protein with fast protein maturation and high stability at a wide range of pH (pH 4 – 12). copGFP does not require any additional cofactors or substrates and has very bright fluorescence that exceeds at least 1.3 times the brightness of EGFP, the widely used Aequorea victoria GFP mutant. The copGFP protein emits green fluorescence with the following characteristics:

• Maximum emission wavelength: 502 nm
• Maximum excitation wavelength: 482 nm
• Quantum yield: 0.6
• Extinction coefficient: 70,000 M^-1 cm^-1

美国SBI代理 System Biosciences,简称SBI，美国加州湾区新成立的生技公司，致力于独特、创新生物技术之开发，以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。 现阶段研发重心为RNA干扰（RNAi）研究之相关工具。 System Biosciences (SBI) 致力于开发独特、革新的技术，为客户研究蛋白组学和基因组学功能提供研究工具。SBI 是专业的慢病毒产品公司，提供基于慢病毒 美国SBI代理 ExoQuick and ExoQuick-TC Products Product Size Catalog # ExoQuick serum exosome precipitation solution (5 ml) 75 reactions EXOQ5A-1 ExoQuick Plasma prep and Exosome precipitation kit (5 ml ExoQuick plus 500 ul Thrombin at 500U/mL), replaces EXOQ5TD-1 product 75 reactions EXOQ5TM-1 Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL), replaces TDEXO-1 product 100 reactions TMEXO-1 ExoQuick serum exosome precipitation solution (20 ml) 300 reactions EXOQ20A-1 ExoQuick-TC for Tissue Culture Media and Urine (10ml) 10 reactions EXOTC10A-1 ExoQuick-TC for Tissue Culture Media and Urine (50ml) 50 reactions EXOTC50A-1 Exosome-depleted FBS Media Supplement - USA Certified 50 ml EXO-FBS-50A-1 Exosome-depleted FBS Media Supplement - USA Certified 250 ml EXO-FBS-250A-1 ExoQuick-LP Lipoprotein Pre-Clear & Exosome Precipitation Kit 5 reactions EXOLP5A-1